Part:BBa_K108024

RuvC

component of RuvABC resolvasome, endonuclease, of E.coli

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Information contributed by City of London UK (2021)

Part information is collated here to help future users of the BioBrick registry.

Metadata:

Group: City of London UK 2021
Author: Haotong Xiong
Summary: Added information collated from existing scientific studies

RuvC is a nuclease that resolves Holliday junction intermediates in genetic recombination. ^[1]

It cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.

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↑ Iwasaki, H., M. Takahagi, T. Shiba, A. Nakata, and H. Shinagawa. 1991. “Escherichia Coli RuvC Protein Is an Endonuclease That Resolves the Holliday Structure.” The EMBO Journal 10 (13): 4381–89. https://pubmed.ncbi.nlm.nih.gov/1661673/.
↑ Dunderdale, H. J., F. E. Benson, C. A. Parsons, G. J. Sharples, R. G. Lloyd, and S. C. West. 1991. “Formation and Resolution of Recombination Intermediates by E. Coli RecA and RuvC Proteins.” Nature 354 (6354): 506–10. https://doi.org/10.1038/354506a0.

[1] Iwasaki, H., M. Takahagi, T. Shiba, A. Nakata, and H. Shinagawa. 1991. “Escherichia Coli RuvC Protein Is an Endonuclease That Resolves the Holliday Structure.” The EMBO Journal 10 (13): 4381–89. https://pubmed.ncbi.nlm.nih.gov/1661673/.

[2] Dunderdale, H. J., F. E. Benson, C. A. Parsons, G. J. Sharples, R. G. Lloyd, and S. C. West. 1991. “Formation and Resolution of Recombination Intermediates by E. Coli RecA and RuvC Proteins.” Nature 354 (6354): 506–10. https://doi.org/10.1038/354506a0.

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